Neuronal cell labelled with anti MAP2 antibody - sample image for software testing

<p><strong>Hippocampal neurons</strong> in culture seeded on glass coverslip. Dendritic extensions are labeled with antibody against <strong>MAP2, a protein associated with microtubules and nucleus is labeled with DAPI</strong>. The resulting signal give acces to the neuronal cell body and dendrite. Here the axon is not labeled. This picture has been taken on a Leica DMRE microscope with a 40x objective. This is a sample demo to be used to test our automatic segmentation of neuronal cells thanks to the <strong>Easy cell shape protocol</strong>, available freely on Icy software. You can download the software (PC, Mac or linux) and have access to the full documentation of this solution on <a href="http://icy.bioimageanalysis.org/protocol/easy-cell-shape-with-hk-means/">http://icy.bioimageanalysis.org/protocol/easy-cell-shape-with-hk-means/ (</a>Publication ID: ICY-77638). It segments in a user friendly mode the cell shape using the hk-mean plugin that applies a N-class thresholding based on a K-Means classification of the image histogram.</p>