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Supplementary data for calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization

<p><strong>Supplementary datasets for the paper&nbsp;Calcium-vesicles perform active diffusion in the sea urchin embryo during larval biomineralization.</strong></p> <p>Two deskewed and deconvolved lattice light-sheet datasets (100 frames each) from the live-cell experiments are available, a control embryo dataset (01-07-2016_TimeLapse4_DMSO_21hrs_Calcein_FM464) and a VEGFR inhibited dataset (24-06-2016_Timelapse1_Axtinib_150_19hrs_Calcein_FM464). These datasets were used for collecting size and motion statistics. The control embryo dataset is available in raw microscope output without deskew or deconvolution applied (Raw_01-07-2016_TimeLapse4_DMSO_21hrs_Calcein_FM464).</p> <p>Four confocal datasets from the cytoskeletal remodeling experiments are also included, phalloidin stained images, control (Phalloidin PMC DMSO 5 zoom4s) and VEGFR inhibited (Phalloidin PMC Axt 18 zoom4); and myosinIIP stained images, control (Myosin PMC 30h DMSO new slid 4a zoom4) and VEGFR inhibited (Phalloidin PMC Axt 18 zoom4).</p> <p>Source code and instructions for the analysis tools used for both the lattice light-sheet and confocal data is available at: <a href="https://git-bioimage.coe.drexel.edu/opensource/llsm-calcium-vesicles-lever">https://git-bioimage.coe.drexel.edu/opensource/llsm-calcium-vesicles-lever</a></p> <p>Code for the deconvolution and deskew algorithms is available from the Janelia research center at: <a href="https://www.janelia.org/open-science/lattice-light-deconvolution-software-cudadeconv">https://www.janelia.org/open-science/lattice-light-deconvolution-software-cudadeconv</a></p> <p>&nbsp;</p> <p>&nbsp;</p>

ShareScore

40/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
8
Harmonization
4
Access
16
Reuse readiness
8
Engagement
4

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