4 micron sections of human thyroid cancer stained with PGP9.5 and counterstained with haematoxylin

<p>Whole slide sections of 4um thickness, from formalin-fixed paraffin embedded blocks of benign and malignant thyroid tissue were labelled with immunohistochemistry for the pan-neuronal marker protein gene-product 9.5 (PGP9.5) using the Ventana Discovery automated slide stainer (Roche Medical Systems, Tuscon, Az), then counterstained with haematoxylin. The primary antibody was anti-rabbit polyclonal PGP9.5 antibody (catalogue number #Ab15503, Abcam, Cambridge, United Kingdom) at 1:600 dilution. Slides were then digitized at 20 X magnification using the Aperio AT2 scanner (Leica Biosystems, Victoria, Australia).&nbsp; The Hunter New England Ethics Committee has approved a subset of the dataset to be publicly available in fully deidentified format for the purpose of validating models for automated detection of nerve tissue.&nbsp; No demographic or clinical details are provided.&nbsp;</p>