Gradients2-MGL1704-HPLC_2020-03-25_v1.0
<p>Cruise: Gradients 2, MGL1704</p> <p>Project Name: Simons Foundation Gradients 2 NPSG</p> <p>Description: Samples were processed to detect and quantify phytoplankton pigments using a modified version of the high-performance liquid chromatography (HPLC) method of Wright et al. (1991) as described by Bidigare et al. (2005). HPLC samples were collected by semi-automated and sequential filtration of the ship's underway flow through seawater onto Whatman GF/F filters (0.7 µm nominal pore size) and stored at -80°C until analysis. Filters were extracted in 100% acetone for 24 h at 4°C. Extracts were processed in reverse-phase on a 250 mm C18 column using a Waters separations module and photodiode detector array. Empower chromatography software was used for integration and pigment quantification. The instrument was calibrated using chlorophyll a from Anacystis nidulans (Sigma USA) and mixed pigment standards were obtained from DHI in Denmark.</p>
ShareScore
36/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 4
- Harmonization
- 4
- Access
- 16
- Reuse readiness
- 8
- Engagement
- 4
ScienceDex for faculty
Your academic CV and NIH biosketch
Make your CV in your school's format and your NIH biosketch from it, then download both as Word files.