Fluorescent Membrane Tension Probes for Early Endosomes

<p><strong>Table of contents</strong></p> <p>1. Synthesis and Characterization</p> <p>1.1. Compounds <strong>1</strong>,<strong> 3</strong>,<strong> 4</strong>,<strong> 6</strong>,<strong> 7</strong>,<strong> 9</strong>,<strong> 10</strong>, <strong>11</strong>,<strong> 12</strong></p> <ul> <li>Lab Book Page</li> <li>NMR</li> <li>LC-MS</li> <li>IR</li> <li>HRMS</li> </ul> <p>1.2. Compounds <strong>15</strong>,<strong> 17</strong>,<strong> 19</strong>,<strong> 24</strong>,<strong> 1&rsquo;</strong>,<strong> 3&rsquo;</strong>,<strong> 5&rsquo;</strong>,<strong> 7&rsquo;</strong></p> <ul> <li>Lab Book Page</li> <li>NMR</li> <li>LC-MS</li> <li>IR</li> <li>ESI-MS</li> </ul> <p>1.3. Compounds <strong>21</strong>,<strong> 23</strong>,<strong> 26</strong>,<strong> 8&rsquo;</strong>,<strong> 10&rsquo;</strong></p> <ul> <li>Lab Book Page</li> <li>NMR</li> <li>LC-MS</li> <li>IR</li> </ul> <p>2. p<em>K</em>_a Determination</p> <p>2.1. Titration of <strong>32</strong>,<strong> 35</strong>,<strong> 36</strong> for the determination of the correction function</p> <ul> <li>Lab Book Page</li> <li>NMR</li> </ul> <p>2.2. Titration of Model Compounds <strong>1&rsquo;</strong> &ndash; <strong>10&rsquo;</strong></p> <ul> <li>Lab Book Page</li> <li>NMR&nbsp;</li> </ul> <p>3. Co-Localization with EGF</p> <p>4. Automated Microscopy</p> <p>5. FLIM Imaging</p> <p>6. FLIM of Early Endosomes</p>