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Scripts and raw data for comparing 2D vs 3D image analysis of zebrafish embryo microscopic data

<p>Raw data and MatLab&nbsp;scripts&nbsp;used for image analysis of RNA polymerase II with serine 5 phosphorylation in the C-terminal domain (CTD) of the subunit 1 (Pol II Ser5P) in a fixed&nbsp;zebrafish embryo, comparing a 2D vs 3D approach to segment out the Pol II Ser5P clusters.&nbsp;Pol II Ser5P was labeled by immunofluorescence, microscopy images were acquired by instant-SIM microscopy&nbsp;and analyzed using MatLab scripts and the bioformats importer.</p>

ShareScore

32/100

Overall dataset sharing score

Score breakdown

These five areas show where the dataset supports — or may limit — practical reuse.

Stewardship
4
Harmonization
4
Access
16
Reuse readiness
8
Engagement
0

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