Verification of CcpA gene knockout

<p>To verify whether the <i>CcpA</i> gene was successfully deleted, we designed the primer for PCR. The primer was designed across the upstream and downstream sequences of the <i>CcpA </i>gene, with a size of about 1827bp. Results showed that two (1827bp and 1985bp) PCR products (WT2 and KO2) were produced, then the amplified DNA products of WT2 and KO2 were electrophoresed on a 1% (wt/vol) agarose gel, and sequenced by Shanghai Sangon Biological Engineering Technology and Services Co., Ltd. for further verification. The results of DNA sequencing confirmed that the <i>CcpA </i>deletion strain was successfully constructed.</p>