MiL-FISH: Multi-labelled oligonucleotides for fluorescence in situ hybridisation improve visualization of bacterial cells

<p>Epifluorescence images of MiL-FISH labelled microorganisms: A) <em>Beggiatoa sp. </em>hybridised with Gam42a, B) <em>Desulfococcus biacutus </em>with DSS658, C) <em>Roseobacter sp. </em>with Ros537, D) <em>Sulfurimonas denitrificans </em>with EPSY914, E) <em>Rhodopirellula sp. </em>SH1 T with PLA46, F) <em>Gramella forsetii </em>with CF319a, G) <em>Metallosphaera sedula </em>with Arch915, H) Composite image of all seven microbial partners in an artificial mix. Letters correlate to images of individual organisms A-G. Scale bar: A &amp; H = 10 &micro;m, B-G = 5 &micro;m.</p> <p>From:</p> <p>Schimak MP, Kleiner M, Wetzel S, Liebeke M, Dubilier N, Fuchs BM. 2015. MiL-FISH: Multi-labelled oligonucleotides for fluorescence in situ hybridisation improve visualization of bacterial cells. Applied and Environmental Microbiology. Accepted manuscript posted online. doi:10.1128/AEM.02776-15</p>