Molecular architecture of the 40S⋅eIF1⋅eIF3 translation initiation complex

<p>Eukaryotic translation initiation requires the recruitment of the large, multiprotein eIF3 complex to the 40S ribosomal subunit. Using X-ray structures of all major components of the minimal, six-subunit <em>Saccharomyces cerevisiae</em> eIF3 core, together with cross-linking coupled to mass spectrometry, we were able to use IMP to position and orient all eIF3 components on the 40S•eIF1 complex, revealing an extended, modular arrangement of eIF3 subunits.</p> <p>For more information about how to reproduce this modeling, see https://salilab.org/40S-eIF1-eIF3 or the README file.</p>