FIGURE 9. DNA fragments amplified from rDNA ITS 1 in Differentiation of larvae and pupae of aquatic genera of Nearctic Hemerodromiinae (Diptera: Empididae)

FIGURE 9. DNA fragments amplified from rDNA ITS 1 primers for the following Hemerodromiinae: 1 = Hemerodromia sp. pupa, 2 = Hemerodromia burdicki adult male, 3 = Neoplasta sp. larva, 4 = Neoplasta sp. pupa, 5 = Neoplasta paramegorchis adult male, 6 = Metachela sp. larva, 7 = Metachela sp. pupa, 8 = Metachela collusor adult male, 9 = Chelifera sp. larva, 10 = Chelifera sp. pupa, 11 = Chelifera caliga adult male. Electrophoretic gel is 1.5% agarose with 100 base pair markers. For reference, numbers 3-5 = ca. 300 base pairs and numbers 6-8 = ca. 500 base pairs.