FIGURE 1. Cox1 in Pachybrachis holerorum (Coleoptera: Chrysomelidae: Cryptocephalinae), a new species from the Apennines, Italy, identified by integration of morphological and molecular data

FIGURE 1. Cox1 maximum likelihood ultrametric rooted tree obtained by GMYC analysis depicting the seven identified species of Pachybrachis (in red). The outgroup (Cryptocephalus zambanellus) and all branches with 0 length were removed in order to perform the analysis (see Materials and Methods). The vertical blue line depicts the estimated threshold between and within species, with the highest value of maximum likelihood; the vertical black line depicts the lowest value of the identified maximum likelihood confidence interval.