Resazurin-based time-kill assay in Acinetobacter baumannii AB074. Fluorescence measurement data for excitation 544 nm, emission 590 nm, gain 1000.

<p>There were 21 groups: 3 groups of transferrin alone in 3 different concentrations (1/3, 1, and 3 times of its MIC); 4 groups of antibiotics alone (ciprofloxacin and meropenem in 1/3 and 1 MIC concentration each), 6 combo groups for ciprofloxacin and 6 combo groups for meropenem, 1 group of positive control with bacteria but without any drug and 1 group of negative control without bacteria, with RPMI only. 20 µL of 0.1% aqueous resazurin solution was added to each well and the plate was incubated at 37°C without shaking for 24 hours. After incubation fluorescence was measured (excitation 544 nm, emission 590 nm) at 0h, 1h, 2h, 4h, 6h, 8h, and 24 hrs using the FLUOstar Omega plate reader (BMG LABTECH GmbH, Germany). Plate layouts may vary.</p> <p>For more information contact author.</p>