Microscopy image data of a titration series with DNA surface strands and condensate-forming DNA nanomotifs (repeat)

<p>The dataset contains microscopy image data of DNA-nanomotifs (color channel 2) that can form liquid-like condensates, especially in association with target DNA strands that can act as condensation surfaces (color channel 1). The data were recorded on a VisiTech iSIM microscopy with a 40X water immersion objective&nbsp;(Apo LWD 40x WI&nbsp;&lambda;S DIC N2,&nbsp;NA 1.15). Images were recorded using dual ORCA-Flash4.0 V3 cameras with simultaneous&nbsp;two-channel acquisition to avoid displacement of objects due to consecutive acquisition of&nbsp;color channels. The file names contain the flow channel number, the presence of condensation surfaces (SX4), and the amount of added nanomotif solution in microliters (NX in &micro;l).</p> <p>The image files are in the .nd2 Nikon proprietary format, but can be read with the OME bioformats libraries.</p> <p>Scripts for the analysis of these data are available via a GitHub repository: <a href="https://github.com/lhilbert/SurfacesAndCondensates" target="_blank" rel="noopener">https://github.com/lhilbert/SurfacesAndCondensates</a></p> <p>This repository contains data recorded on 10 March, 2023</p> <p>The file size of data were too large, so additional repositories were opened. The complete date need to be also downloaded from these additional repositories:</p> <p>EXTRA DATA 1/2: &nbsp;10.5281/zenodo.13788977</p> <pre><a href="https://doi.org/10.5281/zenodo.13788977" target="_blank" rel="noopener">https://doi.org/10.5281/zenodo.13788977</a></pre> <p>EXTRA DATA 2/2: 10.5281/zenodo.13789555</p> <pre><a href="https://doi.org/10.5281/zenodo.13789555" target="_blank" rel="noopener">https://doi.org/10.5281/zenodo.13789555</a></pre> <pre>&nbsp;</pre> <p>There is another data set that contains a repeat of the same conditions, recorded on 13 March, 2023, under the following link</p> <pre><a href="https://doi.org/10.5281/zenodo.13788484" target="_blank" rel="noopener">https://doi.org/10.5281/zenodo.13788484</a></pre> <p><br>From these data sets, two concentration conditions were excluded from further analysis, as described in the file <a href="https://zenodo.org/api/records/15231478/draft/files/Zenodo_Titration_Exclusions.pdf/content" target="_blank" rel="noopener noreferrer">Zenodo_Titration_Exclusions.pdf</a></p>