Data from Nicolle et al. LC-HRMS study of Streptomyces sp. AgN23 Culture Media Extract. Study of AgN23 exometabolome and analysis of Arabidopsis metabolomic responses to the bacteria
<p>This archive compiles several datasets related to studies of <i>Streptomyces</i> sp. AgN23 interaction with <i>Arabidopsis thaliana</i>. Ultra-high-performance liquid chromatography-high-resolution MS (UHPLC-HRMS) analyses were performed on a Q Exactive Plus quadrupole (Orbitrap) mass spectrometer, equipped with a heated electrospray probe (HESI II) coupled to a U-HPLC Ultimate 3000 RSLC system (Thermo Fisher Scientific, Hemel Hempstead, United Kigdom). For each biological sample, the RAW file obtained in ESI+ and ESI- mode were retrieved from the Xcalibur version 4.4 software and are deposited in separate sub-folders termed "RawPos" and "RawNeg". Each experimental cohort is grouped in a folder where the biological repeats can be retrieved, as well as QC (Quality Check, pool of all samples from the cohort), Blank samples and eventual alternative control such as Bennett, the mock control media of <i>Streptomyces</i> sp. AgN23. The details regarding samples preparation, analytic parameters and mass spectrometry, statistical treatment and visualization of the data will be made available in the publication relating to this archive. The folder " AgN23-WT_AgN23-pSC004" contains chromatograms related to metabolomic study of Wild-type and pSC004-1, pSC004-10, pSC004-16 and pSC004-22 mutants of <i>Streptomyces</i> sp. AgN23. The folder " Col-0_AgN23" contains chromatograms related to metabolomic study of <i>Arabidopsis thaliana</i> Col-0 responses to colonization by <i>Streptomyces</i> sp. AgN23-WT. The folder " Col-0_pad3-1_AgN23" contains chromatograms related to metabolomic study of <i>Arabidopsis thaliana</i> Col-0 and the <i>Arabidopsis</i> pad3-1 mutant responses to colonization by <i>Streptomyces</i> sp. AgN23-WT. The folder " Col-0_pSC004" contains chromatograms related to metabolomic study of <i>Arabidopsis thaliana</i> Col-0 responses to colonization by <i>Streptomyces</i> sp. AgN23-WT and the AgN23 mutants pSC004-10 and pSC004-22. It should be noted that in the publication associated with this archive, the pSC004-1, pSC004-10, pSC004-16 and pSC004-22 mutants are referred to as ΔgbnB-1, ΔgbnB-2, ΔgbnB-3 and ΔgbnB-4, respectively.</p>
ShareScore
36/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 8
- Harmonization
- 4
- Access
- 16
- Reuse readiness
- 0
- Engagement
- 8