Figure 1. A in Osmotic stress tolerance in semi-terrestrial tardigrades

Figure 1. A, Light micrograph of Ramazzottius oberhaeuseri. Scale bar = 100 µm. B, Activity in percentage (mean ƚ SEM) of R. oberhaeuseri in experimental series (N = 3–6) with acute exposures to 192 ƚ 1, 463 ƚ 1, 560 ƚ 1 and 758 1 mOsm kg—1 NaCl solutions. The four graph lines (open symbols) the activity in ƚ top express percentage (mean ƚ SEM) of the corresponding control groups (N = 3–6) in purified water. C, Light micrograph of Echiniscus testudo. Scale bar = 100 µm. D, Activity in percentage (mean ƚ SEM) of Echiniscus testudo in experimental series (N = 3–6) with acute exposures to 93 0, 192 1, 281 0 and 463 1 mOsm kg—1 NaCl solutions. The top four graph lines (open ƚ ƚ ƚ ƚ symbols) express the activity in percentage (mean ƚ SEM) of the corresponding control groups (N = 3–6) in purified water. In B and D t = 0 h represents the activity in purified water immediately prior to osmolyte exposure, whereas t = 24 h indicates the activity after exposure to a given test solution for 24 h. The tardigrades were subsequently transferred to purified water and their activity was assessed at t = 26 h, t = 48 h and t = 72 h.