Microchamber slide design for cell confinement during imaging- Tetrahymena rostrata timelapse data

<p>We performed Imaging on a Nikon Ti2-E &amp; Yokogawa CSU W1-SoRa microscope. The microscope was equipped with an ORCA-Fusion BT digital C-MOS camera. We used a 10× 0.45 Plan Apo Air &nbsp;or a &nbsp;Plan Apo λD 100x OIL OFN25 DIC N2 objective for differential interference contrast (DIC) imaging. More microscopy information is detailed in the metadata file associated with each .nd2 file.<br><br><i>Tetrahymena rostrata</i> was provided by Andrzej Kaczanowski, who isolated the cells from the small snail <i>Cohlicopa lubrica</i> near Warsaw. The cells were cultured in medium consisting 0.5% yeast extract and 0.5% of proteose peptone supplemented with 250 ug/ml streptomycin sulphate and 250 ug/ml penicillin G to maintain sterility.</p>