Fig. 5 in Limonoid biosynthesis 3: Functional characterization of crucial genes involved in neem limonoid biosynthesis

Fig. 5. Transient expression of AiTTS1 in neem twig leaves. A) Real-time qPCR analysis showing relative expression levels of AiTTS1 in pRI101-ANand pRI101- AN_AiTTS1 infiltrated leaves. Expression levels of these genes were normalized to actin and are represented incomparison with pRI101-AN control. Data represent means ±SE of two independent biological replicates. B) Limonoid profiling of neem transient leaves between control pRI101-AN and pRI101-AN-AiTTS1. Most of the neem limonoids production is increased as compared to vector control which clearly shows that AiTTS1 involved in limonoid biosynthesis.