NextClone and CloneDetective: An Integrated Nextflow Pipeline and R Package for Clonal Barcode Extraction and Quantification

<p>BAM file (chunk 1-25 of 50) for the scRNAseq data required to replicate the analyses presented at: https://phipsonlab.github.io/NextClone-analysis/.</p><p>Chunk 26-50 can be downloaded from https://zenodo.org/uploads/10129625</p><p>The original BAM file was too big to fit in an entry. Thus it was split into 50 using picard:</p><blockquote><p>SplitSamByNumberOfReads -I possorted_genome_bam.bam -O split_sam/ -N_FILES 50 <i>--CREATE_MD5_FILE</i></p></blockquote><p>Before re-running all the analyses in https://phipsonlab.github.io/NextClone-analysis/, make sure you merge all 50 chunks first using picard (change xxx to point to the directory storing all the 50 chunks you have downloaded):</p><blockquote><p>outdir="xxx"</p><p>args=""</p><p><i># Loop through each BAM file</i></p><p>for file in ${outdir}/*.bam; do</p><p>&nbsp; &nbsp; args+="-I $file "</p><p>done</p><p># Do the actual merging</p><p>MergeSamFiles $args -O $outdir/merged_v2/merged_bam_v2.bam --USE_THREADING --CREATE_MD5_FILE</p></blockquote><p>Picard can be downloaded from: https://github.com/broadinstitute/picard</p>