Data for Altered Glia-Neuron Communication in Alzheimer's Disease Affects WNT, p53, and NFkB Signaling Determined by snRNA-seq

<p><strong>data.tar.gz contains all files from the data directory associated with the 230313_TS_CCCinHumanAD GitHub project and includes the following:</strong></p><ul><li><strong>CellRangerCounts/</strong><ul><li><strong>GSE157827/</strong><ul><li><strong>post_soupX/ : </strong>contains 21 directories for 21 samples, which each contain 3 files obtained from ambient RNA removal with soupX. Below is a representative example, but this repo contains 1 directory per sample:<ul><li><strong>SAMN16100290_S01_AD/</strong><ul><li><strong>barcodes.tsv</strong></li><li><strong>genes.tsv</strong></li><li><strong>matrix.mtx</strong></li></ul></li></ul></li><li><strong>pre_soupX/ : </strong>contains 21 directories for 21 samples, which each contain 2 files obtained from Cell Ranger after aligning fastq files to the reference genome. Below is a representative example, but this repo contains 1 directory per sample:<ul><li><strong>SAMN16100290_S01_AD/</strong><ul><li><strong>filtered_feature_ bc_matrix.h5</strong></li><li><strong>Raw_feature_bc_matrix.h5</strong></li></ul></li></ul></li></ul></li><li><strong>GSE174367/ : </strong>contains 19 directories for 19 samples, which contain 3 files each from Cell Ranger alignment of fastq files to the reference genome. Below is a representative example, but this repo contains 1 directory per sample:<ul><li><strong>SAMN19128610_S1_CTRL/</strong><ul><li><strong>barcodes.tsv</strong></li><li><strong>genes.tsv</strong></li><li><strong>Matrix.mtx</strong></li></ul></li></ul></li></ul></li><li><strong>ccc/</strong><ul><li><strong>nichenet_grn/</strong><ul><li><strong>gr_network_human_21122021.rds : </strong>accessed in October 2023, gene regulation network – gene regulatory information from MultiNicheNet</li><li><strong>ligand_tf_matrix_nsga2r_final.rds: </strong>accessed in October 2023, ligand tf matrix for signaling path determination from MultiNicheNet</li><li><strong>signaling_network_human_21122021.rds : </strong>accessed in October 2023, signaling network – protein-protein interaction information from MultiNicheNet</li><li><strong>weighted_networks_nsga2r_final.rds : </strong>accessed in October 2023, networks weighted by literature evidence from MultiNicheNet</li></ul></li><li><strong>nichenet_prior/</strong><ul><li><strong>ligand_target_matrix.rds : </strong>accessed in April 2023, ligand to target matrix from NicheNet</li><li><strong>lr_network.rds : </strong>accessed in April 2023, ligand-receptor matrix from NicheNet</li></ul></li><li><strong>nichenet_v2_prior/</strong><ul><li><strong>ligand_target_matrix_nsga2r_final.rds : </strong>accessed in June 2023, ligand to target matrix from MultiNicheNet used to predict target genes.</li><li><strong>lr_network_human_21122021.rds : </strong>accessed in June 2023, ligand-receptor matrix from MultiNicheNet used to predict ligand-receptor pairs.</li></ul></li><li><strong>geo_multinichenet_output.rds </strong>: MultiNicheNet output for Morabito et al., 2021 data</li><li><strong>geo_signaling_igraph_objects.rds </strong>: list of igraph objects for 17 overlapping LRTs and their signaling mediators in the Morabito et al., 2021 dataset.&nbsp;</li><li><strong>gse_multinichenet_output.rds</strong> : MultiNicheNet output for Lau et al., 2020 data</li><li><strong>gse_signaling_igraph_objects.rds</strong> : list of igraph objects for 17 overlapping LRTs and their signaling mediators in the Lau et al., 2020 dataset&nbsp;</li></ul></li><li><strong>seurat_preprocessing/</strong><ul><li><strong>geo_filtered_seurat.rds : </strong>merged and filtered seurat object of Morabito et al., 2021 data</li><li><strong>geo_integrated_seurat.rds :</strong> seurat object integrated using harmony of Morabito et al., 2021 data</li><li><strong>geo_clustered_seurat.rds : </strong>clustered seurat object of Morabito et al., 2021 data</li><li><strong>geo_processed_seurat.rds : </strong>processed seurat object with final cell type assignments at specified resolution of Morabito et al., 2021 data</li><li><strong>gse_filtered_seurat.rds : </strong>merged and filtered seurat object of Lau et al., 2020 data</li><li><strong>gse_integrated_seurat.rds : </strong>seurat object integrated using harmony of Lau et al., 2020 data</li><li><strong>gse_clustered_seurat.rds :</strong> clustered seurat object of Lau et al., 2020 data</li><li><strong>gse_processed_seurat.rds : </strong>processed seurat object with final cell type assignments at specified resolution of Lau et al., 2020 data&nbsp;&nbsp;</li></ul></li></ul>