Supplemental Movie 3: Simultaneous dual-color imaging of ICC-SM in the colon and adjacent SMCs.

<p><strong><span>Supplemental Movie 3: Simultaneous dual-color imaging of ICC-SM in the colon and adjacent SMCs.<span>&nbsp;&nbsp; </span></span></strong><span>Video shows spontaneous, propagating Ca²⁺&nbsp;waves through an ICC-SM network along the submucosal surface of the CM in the proximal colon.<span>&nbsp; </span>FOVs are from a muscle of a mouse expressing GCaMP6f in ICC (left FOV; colored green) and RCaMP1.07&nbsp;in SMCs (right FOV; colored red) imaged simultaneously with a 20x objective. The characteristics of the fluorophores are such that there is minimal spectral overlap. Signals in ICC-SM and SMCs are coordinated, showing initiation of each cycle of Ca²⁺&nbsp;transients in the ICC-SM network followed by activation of SMCs adjacent to ICC-SM. Bottom panel shows traces from fluorescence images:<span>&nbsp; </span>ICC-SM transients (green trace) preceded Ca²⁺&nbsp;signals in SMCs (red trace). Copied with permission from Reference </span><span><span>(133)</span></span><span>.</span></p> <p><strong><span>&nbsp;</span></strong></p>