Supplemental Movie 2: Clustered Ca2+ transients (CTCs) in gastric ICC-MY occur from multiple firing sites.

<p><strong><span>Supplemental Movie 2: Clustered Ca²⁺ transients (CTCs) in gastric </span><span>ICC-MY occur from multiple firing sites</span></strong><span>.<span>&nbsp; </span>ICC-MY in the gastric antrum firing of CTCs and imaged at high resolution with a spinning disk confocal microscope using a 60x objective. </span><span>Ca</span>^{<span>2+</span>}<span><span>&nbsp;</span></span><span>signals were monitored in a gastric muscle from a mouse with the genetically encoded </span><span>Ca</span>^{<span>2+</span>}<span><span>&nbsp;</span></span><span>indicator, GCaMP6f, expressed exclusively in ICC. The left panel shows typical stellate-shaped ICC-MY with multiple interconnecting processes. The middle panel shows the </span><span>Ca</span>^{<span>2+</span>}<span><span>&nbsp;</span></span><span>particle (PTCL) activity, color coded in blue for raw PTCLs, and the centroids of particles are indicated in purple and green indicates </span><span>Ca</span>^{<span>2+</span>}<span><span>&nbsp;</span></span><span>firing sites. There are multiple sites firing </span><span>Ca</span>^{<span>2+</span>}<span><span>&nbsp;</span></span><span>transients during the CTCs.<span>&nbsp; </span>The right panel shows an occurrence map of color-coded initiation/firing sites. The pattern of firing sites </span><span>Ca</span>^{<span>2+</span>}<span><span>&nbsp;</span></span><span>activity was temporally clustered as activation of </span><span>Ca</span>^{<span>2+</span>}<span><span>&nbsp;transients </span></span><span>swept through the network of ICC-MY.<span>&nbsp; </span>The onset of the CTCs was explosive, and then asynchronous firing of occurred at multiple sites and was sustained for more than 2 sec.<span>&nbsp; </span>Note also the complete quiescence of firing immediately upon conclusion of a CTC (absolute refractory period) and then sporadic initiation of firing with time.<span>&nbsp; </span>It is the re-initiation of firing that sets off the next CTC by activating ANO1 channels, depolarization and activation of voltage-dependent Ca²⁺ current (see text for details).<span>&nbsp; </span>Reformatted with permission from reference </span><span><span>(106)</span></span><span>.<span>&nbsp; </span></span></p>