The HIFI reads and assembled contigs used to construct the mitochondrial genome assembly of Glyphodes pyloalis

<p><span>Glyphodes pyloalis (Lepidoptera; Crambidae; Spilomelinae), known as the mulberry pyralid, is a notorious insect pest belonging to the Lepidopteran that threatens mulberry cultivation across China. <span>The mitochondrial genome is an invaluable genetic resource owing to its maternal inheritance, rapid evolution, and lack of recombination. A previous study documented the G. pyloalis mitochondrial genome (mtDNA) as NC_025933 using short-read next-generation sequences that, while pioneering, leaves room for improvement with modern long-read sequencing. PacBio&rsquo;s high-fidelity circular consensus sequencing (HIFI CCS) generates extra-long sequences sufficient to produce complete mtDNA assemblies with base pair accuracy.</span></span></p>