Fig. 4 in Cytotoxic cardenolides from Calotropis gigantea

Fig. 4. (A, C) A172 cells were exposed to 0, 50 or 100 nM of 8 or 10 for 24 h. PI staining was used to distinguish the cell cycle distribution of different treatment groups. Flow cytometry was used to analyse the respective cell cycle distribution after staining. (B, D) Statistical analysis representation of the percentage of the three cell cycles (G0/G1, S, and G2/M) analysed by flow cytometry. Error bars were calculated from three independent experiments. *P <0.05; **P <0.01; ***P <0.001 vs. Control.