Fig. 5 in Undescribed isoquinolines from Zanthoxylum nitidum and their antiproliferative effects against human cancer cell lines

Fig. 5. Effects of compound 2b on the cell viability, colony formation and cell cycle distribution in the A549 cells. (A) Cells were treated with compound 2b (0.05, 0.1, or 0.2 μM) for 48 h, and then morphological changes were detected by phase-contrast microscopy (200 × magnification). (B) Cells were treated with 2b (0.05, 0.1, or 0.2 μM) for 14 days. Colony formation was subsequently observed under light microscopy (40 × magnification). (C) Cells were treated with 2b for 24 h, and then the cell cycle distribution was analyzed by flow cytometry. (D) Cells were treated with compound 2b for 24 h, and then the protein expressions of Cyclin A, Cyclin B1, Cdc25C and p21 were detected by western blot analysis. Relative intensity of indicated proteins was quantified using NIH ImageJ software (bottom panel).