Fig. 4 in Phytochemical genomics of the Madagascar periwinkle: Unravelling the last twists of the alkaloid engine

Fig. 4. Virus-induced gene silencing monitored in C. roseus by targeting phytoene desaturase (PDS). (A) Control plant. (B) Photobleaching phenoptype of upper leaves as a marker of PDS silenced tissues in plant co-infiltrated with Agrobacterium tumefaciens strain GV3101 containing pTRV1 and pTRV2-PDS (T-DNA vectors carrying, respectively RNA1 and RNA2 TRV genome) (S. Besseau and V. Courdavault, personal data). Seedlings were grown at 28 °C and transferred at 21 °C after transformation to improve VIGS efficiency as suggested by Sung et al. (2014).