Fig. 5. C2C12 in Insulin-mimetic activity of 23-glycosyl oleanane triterpenoids isolated from Gymnema latifolium

Fig. 5. C2C12 myotube cells were exposed to compounds 2 and 4 (5 μM and 20 μM, respectively) and incubated for 1 h. The phosphorylation of the protein AMPK in the cells was assessed by Western blot analysis. Fold expression was calculated as p-AMPK/AMPK and normalized to the protein level of β-actin. Values are expressed as the mean ± SD (n = 3).