Fig. 4 in Increase in isoflavonoid content in Glycine max cells transformed by the constitutively active Ca independent form of the AtCPK1 gene

Fig. 4. Expression of the G. max genes (a) - of the upstream enzymes - 4- coumarate:CoA ligase (4CL), (b) – of the downstream enzymes - isoflavones synthase (IFS) and the final step of daidzein and genistein biosynthesis - 2- hydroxyisoflavanone dehydratase (HID) and (c) - enzymes involve in prenilation of daidzein and coumestrol - isoflavone dimethylallyltransferase (IDT1) and coumestrol 4-dimethylallyltransferase (C4DT) in the control callus culture (Gm) and callus line transformed with the constitutively active AtCPK1 (GmCa1 and GmCa2). Data (mean ± standard error) represent measurements of three independent replicates from two different RNA isolations and are presented as relative expression levels normalized to the expression of the G. max housekeeping genes. Different letters above the bars indicate statistically significant differences of means (P <0.05), Fisher's LSD.