Refractive index tomography of chitin-containing bristles (chaetae) from Platynereis dumerilii

<p>This dataset is a supplementary material including refractive index tomograms of an article&nbsp;</p> <p>Ikeda, K., Belevich, I., Zelaya-Lainez, L., Orel, L., F&uuml;ssl, J., Gumulec, J., Hellmich, C., Jokitalo, E., Raible, F.: Dynamic microvilli sculpt bristles at nanometric scale. Nature Communications 2024.</p> <p><strong>Biochemical isolation of bristles and imaging</strong></p> <p>Larvae were washed twice using artificial sea water. The larvae were washed in artificial seawater supplemented with 25mM EGTA, then lysed for 1 hour in Triton X-100 1% in the same buffer on ice. We could observe the appearance of a gel containing bristles. The gel was washed twice in 10 mM Tris-HCl, 2.5 mM MgCl2, 0.5 mM, CaCl2pH 7.6. 50 &mu;L were left inthe sample at the last wash and the gel was digested with 5 U of DNase I (Thermo Fischer Scientific) for 2.5 hours at 37&deg;C with 360 rpm agitation. Next,the digest was supplemented with 100 &mu;L of artificial sea water and digested with 200 &mu;L of 2.5 &mu;g/&mu;L Liberase Enzyme Blend (Sigma-Aldrich) for 1 hour at 50&deg;C. The bristles were washed 3 times in artificial seawater. The isolated bristles were mounted on&mu; Slide I Luer (Ibidi). The refractive index tomograms of isolated bristles were obtained by Nanolive 3D Cell Explorer with 60X magnification.</p> <p>The accompanied TIFF is 32-bit, where pixel values correspond to refractive index (e.g. pixel value in image 1.335 = refractive index&nbsp;<em>n</em>). The pixel size is 0.182um,&nbsp; the Z step is 0.372 um.</p>