MASLD metabolomics -- SWATH -- HILIC -- Metabolomics -- Negative mode

<p>We analyzed liver tissues, liver secretome (conditioned medium), and blood plasma samples from 109 obese patients undergoing bariatric surgery spanning early stages of metabolic dysfunction-associated steatotic liver diseases (MASLD). We used a combination of data-dependent acquisition (DDA) and data-independent acquisition (DIA or SWATH-MS) on SCIEX TripleTOF 6600.</p> <p>Metabolites and lipids were extracted using modified chloroform-methanol biphasic extraction methods for both biological matrices. Organic and aqueous fractions were separated using RRHD Eclipse Plus C18 and SeQuant ZIC-cHILIC columns prior to DDA and DIA acquisitions, respectively.</p> <p>All mzML files are deposited with ZENODO.</p> <p>*** HILIC - Polar metabolite - Negative mode - SWATH&nbsp; is deposited in this dataset repository ***</p>