Cryo soft X-ray tomography of 5 microns thick slab extracted from C. elegans midbody region

<p>Samples of C. elegans&nbsp;<span>larvae of strain DM8005&nbsp;</span>were high pressure frozen using the waffle method. Multiple&nbsp;<span>roughly&nbsp;</span>5 micron thick slabs spanning&nbsp;<span>an L2 larva</span>&nbsp;were extracted using&nbsp;<span>the Serial Lift-Out</span>&nbsp;technique, and placed on rectangular&nbsp;<span>mesh&nbsp;</span>grids. Soft X-ray tomography was performed on individual slabs using the SXT-100 soft x-ray microscope at SiriusXT. The data reveals label free internal structure of fully hydrated C. elegans tissue, annotating the worm volume and adding context to cryo electron tomography data.&nbsp;</p> <p>SXT data was acquired from -50&deg; to +53&deg; in 1&deg; steps and reconstructed using Weighted Back Projection. A 32 nm objective zone plate was used. Data pixel size is 29 nm.&nbsp;</p>