Mar2025 reference datasets for Mitohelper

<p><strong>Reference datasets (Mar 2025 update) for Mitohelper (</strong><a href="https://github.com/aomlomics/mitohelper"><em><strong>https://github.com/aomlomics/mitohelper</strong></em></a><strong>)</strong></p> <p><a href="http://github.com/aomlomics/mitohelper">Mitohelper</a>&nbsp;is a repository built to facilitate experimental design, alignment visualization, and reference sequence analysis in fish eDNA studies. Refer to our&nbsp;<a href="https://doi.org/10.1002/edn3.187">paper</a>&nbsp;and&nbsp;Mitohelper's <a href="https://github.com/aomlomics/mitohelper/wiki">wiki</a> for database construction pipeline.</p> <p>I. Reference database files in tab-separated format, containing gene, taxonomy, and sequence information:</p> <ul> <li>mitofish.all.Mar2025.tsv (883,519 records)</li> <li>mitofish.12S.Mar2025.tsv (61,379 records of 12S rRNA gene &gt;50 bp long)</li> <li>mitofish.12S.Mar2025_NR.fasta (FASTA file of 12S rRNA gene records)</li> <li>mitofish.COI.Mar2025.tsv (323,337 records)</li> </ul> <p>II. De-replicated QIIME 2-compatible 12S/12S+16S+18S rRNA reference datasets:</p> <ul> <li>12S-seqs-derep-uniq.qza</li> <li>12S-tax-derep-uniq.qza</li> <li>12S-16S-18S-seqs.qza</li> <li>12S-16S-18S-tax.qza</li> </ul> <p>If you use Mitohelper, please cite:<br>Jean Lim, S, Thompson, LR. Mitohelper: A mitochondrial reference sequence analysis tool for fish eDNA studies. Environmental DNA. 2021; 00: 1&ndash; 10. &nbsp;<a href="https://doi.org/10.1002/edn3.187">https://doi.org/10.1002/edn3.187</a></p>