Supplementary material S31: Averaged spectrograms for the full collection of jolting pulses on each substrate.

<p>A series of spectrograms to demonstrate the filtering parameters chosen for each collection of jolt pulses to improve the alignment process. A selection of pulses from each substrate collection were averaged to produce each panel. This number differed based upon the number of pulses that showcased clarity (petri-dish = 40, honeycomb = 8, brood-comb = 40). Background frequencies were filtered as follows: 19 kHz high-pass (a), 500 Hz high-pass and 12 &ndash; 19 kHz band-stop (b), 500-2500 Hz bandpass (c), leaving only the frequencies where strong signal occurs. This then produces cleaner accelerometer data for improved scrutiny of the pulses to identify similarities within each substrate. The colour-coding of each spectrogram is not relevant for this figure as it serves only the purpose of substantiating the filtering choices.&nbsp;</p>