Supplementary material S20: Instances of jolting pulses with double-peaks, compared to those with single peaks.

<p>A video to demonstrate a double peak jolt and single peak jolt in quick succession. Video excerpts taken from the brood-comb jolting video (S10) here show instances of a double peak jolt with instances of a single peak jolt. The video and audio are slowed four times both for easier viewing and hearing of the jolting vibration, which is here heard as a &lsquo;knocking&rsquo; sound. A double peak jolt can first be seen near to the 29.655 min time stamp on the x axis, immediately followed by a single peak jolt near to the 29.665 min time stamp. The difference in body motion can be best seen when viewing these jolting instances side-by-side. For the double peak, the mite appears to move its body right and then left, whereas for the single peak the body only moves to the right. This is immediately followed by two consecutive instances of single jolts at time stamps 30.3 min and 30.315 min time stamps where the body only moves in a single downward motion. A second instance of a double peak can then be seen at the 31.84 min time stamp, where the mite appears to move the body in a downward motion followed by movement to the right. Two audible &lsquo;knocks&rsquo; can be clearly heard for the double peaks, in comparison to the one audible &lsquo;knock&rsquo; for the single peaks.</p>