Supplementary material S11: Video analysis of Varroa jolting pulses on honeycomb.

<p>Video showing the accelerometer data in spectrogram format and synchronous displacements of the <em>Varroa</em> individual on empty, fully built honeycomb. The processing for all panels in this video has been completed in the same way as for S9 and S10, with the video slowed to four times for ease of viewing. The lowest point of acceleration magnitude is here forced to be 1/10^th of the maximum. The mite in this video pulses 28 times over a period of approximately 4 minutes, so the frame rate did not need to be slowed down to 5 frames per second as with S8, where jolting pulses are rapid and continuous. Due to the less regular production of pulses, there are many stretches of time where no jolting pulses occur. To view the synchronicity quickly and easily between mite displacement and accelerometer trace, instances of jolting have been cut together, thereby removing those time periods where no activity occurred. Overall, this video accumulates approximately 24 seconds of mite activity. The soundtrack (i.e. the signal from the accelerometer) has also been included, as in S9 and S10, also slowed accordingly. The jolting pulses can be heard in some instances as a &lsquo;clapping&rsquo; noise.</p>