FIGURES 1–9. Acaulospora fanjing spores. 1. Intact spores. 2. Crushed spore. 3–6 in Acaulospora fanjing (Glomeromycota), a new species of arbuscular mycorrhizal fungi from Fanjingshan in China

FIGURES 1–9. Acaulospora fanjing spores. 1. Intact spores. 2. Crushed spore. 3–6. Spore wall layers (SW+MW+IW), SWL1 smooth, hyaline and evanescent and completely shed; SWL2 and SWL3 laminate and similar color, in some spores the boundaries between layers are difficult to distinguish; Middle wall (MWLl–MWL2) hyaline, semiflexible bilayered tightly adherent; Inner wall (IWL1–IWL3) hyaline, consists of three layers. Note: MW and IW are easily separated in PVLG and IWL2 shows a pale pink reaction to Melzer's reagent. 7–8. Cicatrix circular to subcircular, slightly raised collar. 9. Mycorrhizal structures of A.fanjing in roots of Trifolium repens stained in 0.1% Trypan blue: hyphae (H) and vesicles (V). 3, 4, 6. In Melzer's. 5. In PVLG+Melzer's reagent.