Live-cell fluorescence microscopy data for: Structural Dynamics of the Functional Nonameric Type III Translocase Export Gate

<p><strong>yuan_t3ss_raw_data.zip:</strong></p> <p>Microscopy data types:<br> <strong>- </strong>Brightfield images<br> - Fluorescence images (514 nm excitation)</p> <p>Microscope:<br> Olympus IX-83 with 1.49NA oil-immersion objective (Olympus UAPON 100x)<br> ET442/514/561 Laser triple band set filtercube (69904, Chroma)</p> <p>Camera:<br> Hamamatsu C9100-13 EM-CCD Camera<br> 80 nm pixel size</p> <p><strong>yuan_t3ss_cell_objects.zip:</strong></p> <p>EPEC and C41 ColiCoords cell objects derived from the raw data. The set contains a total of 24979 individual cells (brightfield, binary, fluorescence and localization datasets for each cell) with corresponding coordinate systems.</p> <p>Code to generate these cell objects from the raw data: https://github.com/Jhsmit/T3SS-paper</p> <p>The data format is .hdf5 and can be read with any HDF5 reader or directly with ColiCoords: https://github.com/Jhsmit/ColiCoords</p> <p><strong>Contact:</strong><br> Jochem Smit</p>