Microbiome diversity and reproductive incompatibility induced by the prevalent endosymbiont Arsenophonus in two species of African cassava Bemisia tabaci whiteflies

<p>This dataset contains data from two-part experiments described in the paper: "El Hamss, H., Ghosh, S., M. N., M., Delatte, H., &amp; Colvin, J. (2021). Microbiome diversity and reproductive incompatibility induced by the prevalent endosymbiont Arsenophonus in two species of African cassava Bemisia tabaci whiteflies. Ecology and Evolution, 00, 1–10. https://doi.org/10.1002/ece3.840".</p> <p>The experiment investigates the effects of <em>Arsenophonus</em> on whitefly reproduction and microbiome diversity.</p> <p>In the first experiment ("crossing experiment"), the effect of <em>Arsenophonus </em>is studied on number of eggs, nymphs, males, and females on different whitefly species at intraspecies level (SSA1-SG3A+ with SSA1-SG3A-) and at interspecies level (SSA2A+ with SSA1-SG3A- and SSA1-SG3A+). <em>Arsenophonus </em>infection A+ means the presence of <em>Arsenophonus </em>in related whitefly colony, A- means the absence of <em>Arsenophonus</em>.</p> <p>In the second experiment ("microbiome diversity"), whitefly colonies of crossed parents and generated progenies were sequenced to check <i>Arsenophonus</i> threshold (number of reads) and the presence of other bacteria in those crossed whiteflies.</p> <p>The main results of this work are:</p> <p>(i) Arsenophonus did not induce reproductive incompatibility within SSA1-SG3 but reduced the number of eggs, nymphs and female ratios,</p> <p>(ii) complete RI was observed between SSA1-SG3 and SSA2 indicating the lack of gene flow between the two whitefly species,</p> <p>(iii) many new 'other bacteria' in SSA <em>B. tabaci</em> have been identified, whose role remains to be investigated.</p>