Hyperinsulinemic insulin receptors fail to internalize post-stimulation.
<p>Fig. S1. (A) Representative immunoblots and (B) densitometry analysis of internalized insulin receptors (InsR-β) following 0, 5, 15, and 30 minutes of stimulation in mouse brain microvascular endothelial cells that are untreated (Non-HI) or incubated in experimental hyperinsulinemic conditions for 12 hours (HI). Data was analyzed via two-way ANOVA with Tukey’s multiple comparisons, n= 6-8/ group, N=4; ✱p< 0.05. All images were normalized to total gel protein concentrations shown as “Stain Free Gel”, calculated in Image Lab software per the manufacturer’s instructions. Line down center of immunoblot indicates a break in the blot; images are from the same immunoblot for each figure and received identical formatting. Data from 15 minutes post-stimulation is same as Figure 4 non-HI and HI.</p>
ShareScore
36/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 4
- Harmonization
- 4
- Access
- 16
- Reuse readiness
- 8
- Engagement
- 4