Dataset related to the article: "Cyclooxygenase-2 Glycosylation Is Affected by Peroxynitrite in Endothelial Cells: Impact on Enzyme Activity and Degradation"

<p>This record containsraw data related to the article: &quot;Cyclooxygenase-2 Glycosylation Is Affected by Peroxynitrite in Endothelial Cells: Impact on Enzyme Activity and Degradation&quot;</p> <p>Abstract: The exposure of human endothelial cells to 3-morpholinosydnonimine (SIN-1) induced the<br> expression of cyclooxygenase-2 (COX-2) in a dose- and time-dependent manner. Interestingly, after a<br> prolonged incubation (&gt;8 h) several proteoforms were visualized by Western blot, corresponding<br> to different states of glycosylation of the protein. This effect was specific for SIN-1 that generates<br> peroxynitrite and it was not detected with other nitric oxide-donors. Metabolic labeling experiments<br> using 35S or cycloheximide suggested that the formation of hypoglycosylated COX-2 was dependent<br> on de novo synthesis of the protein rather than the deglycosylation of the native protein. Moreover,<br> SIN-1 reduced the activity of the hexokinase, the enzyme responsible for the first step of glycolysis.<br> The hypoglycosylated COX-2 induced by SIN-1 showed a reduced capacity to generate prostaglandins<br> and the activity was only partially recovered after immunoprecipitation. Finally, hypoglycosylated<br> COX-2 showed a more rapid rate of degradation compared to COX-2 induced by IL-1a&nbsp;and an<br> alteration in the localization with an accumulation mainly detected in the nuclear membrane. Our<br> results have important implication to understand the effect of peroxynitrite on COX-2 expression<br> and activity, and they may help to identify new pharmacological tools direct to increase COX-2<br> degradation or to inhibit its activity.</p>