raw data for Optogenetic Stimulation of Prelimbic Pyramidal Neurons Maintains Fear Memories and Modulates Amygdala Pyramidal Neuron Transcriptome

<p>Figure Legend &nbsp;</p> <p>Modulation of cellular excitability of Prelimbic (PrL) pyramidal neurons by optogenetic stimulation. (<strong>A</strong>) Representative traces in current-clamp configuration reporting evoked firing activity triggered by a series of depolarizing current steps (0 to 400 pA) applied to PrL pyramidal neurons of SHAM FEAR (black,&nbsp;<em>n</em>&nbsp;= 8 neurons from 5 mice), OPTO FEAR (red,&nbsp;<em>n</em>= 8 neurons from 5 mice), and No-EX (green,&nbsp;<em>n</em>&nbsp;= 5 neurons from 3 mice) groups. The cumulative plot shows the changes in firing activity. (<strong>B</strong>) Representative traces of PrL pyramidal neurons of SHAM FEAR (black,&nbsp;<em>n</em>&nbsp;= 8 neurons from 5 mice), OPTO FEAR (red,&nbsp;<em>n</em>= 8 neurons from 5 mice), and No-EX (green,&nbsp;<em>n</em>&nbsp;= 5 neurons from 3 mice) groups showing the firing activity triggered by linear depolarization from 0 to 800 pA. Graph (on the right) reports the effects of optogenetic stimulation on rheobase value. Namely, PrL pyramidal neurons of OPTO FEAR and No-EX groups recorded after optogenetic stimulation showed a clear reduction in the rheobase value in comparison to neurons of SHAM FEAR group (* at least&nbsp;<em>p</em>= 0.01). (<strong>C</strong>) Representative traces of Excitatory Post-Synaptic Currents (EPSC) of PrL pyramidal neurons of SHAM FEAR (black,&nbsp;<em>n</em>&nbsp;= 8 neurons from 5 mice), OPTO FEAR (red,&nbsp;<em>n</em>= 8 neurons from 5 mice), and No-EX (green,&nbsp;<em>n</em>&nbsp;= 5 neurons from 3 mice) groups. Graph plot (in the middle) and cumulative curve (on the right) depict the clear increase in firing frequency in PrL pyramidal neurons of OPTO FEAR and No-EX groups (* at least&nbsp;<em>p</em>&nbsp;= 0.01). (<strong>D</strong>) Graphs and cumulative curves report no significant differences in cellular excitability in PrL pyramidal neurons of SHAM NOT FEAR (black) and OPTO NOT FEAR (blue) groups. Data are reported as median with interquartile range.</p>