Raw data for: "CalDAG-GEFI mediates striatal cholinergic modulation of dendritic excitability, synaptic plasticity and psychomotor behaviors"

<p>Figure 2. CDGI mediates the M1R modulation of dendritic excitability but not the M1R</p> <p>modulation of somatic excitability.</p> <p>(A and B) Sagittal sections through the brains of CDGI knockout mice in which the direct</p> <p>pathway was visualized (red) in D1-tdTomato mice (A) and the indirect pathway was visualized</p> <p>(green) in D2-GFP mice.</p> <p>(C) Sample somatic voltage changes evoked by 120pA current injections in iSPNs from WT</p> <p>(black) and CDGI-KO (red) before and after bath application of oxo-M (10 &micro;M).</p> <p>(C-D) Current-response curves of iSPNs from WT (B, n=5 cells) and CDGI-KO mice (C, n=7</p> <p>cells). Somatic excitability of iSPNs was similarly enhanced by oxo-M in WT and CDGI-KO.</p> <p>(E) Sample somatic recordings in response to 140pA current injections in dSPNs from WT</p> <p>(black) and CDGI KO (red) before and after bath application of oxo-M (10 &micro;M).</p> <p>(F-H) Current-response curves of dSPNs from WT (E) and CDGI-KO (F) mice (n=4-6).</p> <p>(I) Trains of five EPSPs were evoked by stimulation of glutamatergic afferent fibers at 40 Hz.</p> <p>Oxo-M (10 &micro;M) increased EPSP summation in iSPNs of WT, but not in CDGI-KO or when</p> <p>M1Rs were blocked by M1R antagonist VU0255035 in WT (5 M).</p> <p>(J) Box plot showing the effect of oxoM on synaptic summation. The EPSP5/EPSP1 ratio was</p> <p>increased by oxoM in iSPNs of WT (p = 0.002, Wilcoxon test; n = 10), but not in iSPNs of 27</p> <p>CDGI-KO mice (p = 0.25, n = 9) or in iSPNs of WT mice in the presence of VU0255035 (p =</p> <p>0.69, n = 6).</p> <p>(K) Box plot showing the effect of oxoM on the kinetics of synaptic response. The decay time</p> <p>constant of EPSP5 was significantly increased by oxoM in iSPNs of WT (p = 0.002); but not</p> <p>when CDGI was genetically deleted (p = 0.65) or when M1R was pharmacologically blocked (p</p> <p>= 0.84).</p>