Data from: Limited evidence of a genetic basis for sex determination in the common creek chub, Semotilus atromaculatus

<p>Sexual reproduction is almost universal in vertebrates of the animal kingdom; therefore, each species which use it must have a mechanism for designating sex as male or female. Fish especially have a wide range of sex determining systems. In the present study, we aimed to identify a genetic basis for sex determination in the common creek chub (<em>Semotilus atromaculatus</em>) using genotyping-by-sequencing data. No sex-associated markers were found by RADSex or a GWAS using GEMMA, however, our Weir and Cockerham locus-specific F_ST analysis and discriminant analysis of principal components revealed some genetic differentiation between the sexes at several loci. While no explicit sex determination mechanism has been yet discovered in creek chub, these loci are potential candidates for future studies. Incompatible systems are thought to increase reproductive isolation but interspecific hybridization is common among groups such as cyprinid minnows; thus, studies such as ours can provide insight into hybridization and evolutionary diversification of this clade. We also highlight technical challenges involved in studying sex determination in species with extremely variable mechanisms.</p>