Phenotype data for: Pleiotropic and non-redundant effects of an auxin importer in Setaria and maize

<p>Directional transport of auxin is critical for inflorescence and floral development in flowering plants, but the role of auxin influx carriers (AUX1 proteins) has been largely overlooked. Taking advantage of available AUX1 mutants in <i>Setaria viridis </i>and maize, we uncover previously unreported aspects of plant development that are affected by auxin influx, including higher order branches in the inflorescence, stigma branch number, and glume (floral bract) development, and plant fertility.  However, disruption of auxin flux does not affect all parts of the plant, with little obvious effect on inflorescence meristem size, time to flowering, and anther morphology.  In double mutant studies in maize, disruptions of <i>ZmAUX1</i> also affect vegetative development.  A GFP-tagged construct of <i>Spp1</i> under its native promoter showed that the SPP1 protein localizes to the plasma membrane of outer tissue layers in both roots and inflorescences, and accumulates specifically in inflorescence branch meristems, consistent with the mutant phenotype and expected auxin maxima.  RNA-seq analysis finds that most gene expression modules are conserved between mutant and wildtype plants, with only a few hundred genes differentially expressed in <i>spp1</i> inflorescences. Using CRISPR-Cas9 technology, we disrupted <i>SPP1</i> and the other four <i>AUX1</i> homologs in <i>S. viridis</i>.  SPP1 has a larger effect on inflorescence development than the others, although all contribute to plant height, tiller formation, leaf, and root development. The AUX1 importers are thus not fully redundant in <i>S. viridis</i>.  Our detailed phenotypic characterization plus a stable GFP-tagged line offer tools for future dissection of the function of auxin influx proteins.</p>