RPE-1 GFP-H2B iCas9 cells undergoing paclitaxel-induced mitotic arrest
<p>Phasefocus LiveCyte video showing RPE-1 GFP-H2B iCas9 cells undergoing paclitaxel-induced mitotic arrest. Cells were treated with 1 μg/mL doxycycline for 96 hours to induce Cas9 expression and were imaged every 9 minutes for 48 hours at 20x magnification. From 3:17 cells start entering mitosis as indicated by their rounding up into a ball; from 12:44 mitotic cells begin to either slip from mitosis as indicated by rounded mitotic cells flattening or undergoing mitotic cell death as indicated by the presence of propidium iodide (red).</p>
ShareScore
36/100
Overall dataset sharing score
Score breakdown
These five areas show where the dataset supports — or may limit — practical reuse.
- Stewardship
- 8
- Harmonization
- 4
- Access
- 16
- Reuse readiness
- 8
- Engagement
- 0
Topics
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