Rapid evolution of thermal tolerance and phenotypic plasticity in variable environments

<p>These are the data and code to go with &quot;<strong>Rapid evolution of thermal tolerance and phenotypic plasticity in variable environments</strong>&quot;</p> <p><strong>Figure 01 takes the following data/scripts:</strong></p> <p><a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/20210610_Thally02_Figure01_plot_and_stats.R">20210610_Thally02_Figure01_plot_and_stats.R </a>&nbsp;with <a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/track_keeper.csv">track_keeper.csv </a>&nbsp;and <a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/corr_Response%20growth%20.csv">corr_Response growth .csv </a>. These files contain growth rates per transfers for all selection environments throughout the experiment and growth rates in correlated environments during reciprocal transplants, respectively.&nbsp;</p> <p><strong>Figure 02 takes the following data/scripts:</strong></p> <p>&nbsp;</p> <p><a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/20210610_Thally02_Figure02_plot_and_stats.R">20210610_Thally02_Figure02_plot_and_stats.R </a>&nbsp;with&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/20161120_res_logis_t000.csv">20161120_res_logis_t000.csv&nbsp;</a>and&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/20161123_resloglint300.csv">20161123_resloglint300.csv .</a>&nbsp;These files contain the output of the shapes of the growth curves (i.e. information on lag time , growth at &micro;max, K etc) for all samples in all selection environments at t0 and t300, respectively</p> <p><strong>The remaining figures - position not clear at time of submission - take the following data/script.&nbsp;</strong></p> <p>For plasticity in FRRF data, the script&nbsp;20181204_FRRF_plasticity.R takes the FRRF raw data contained in&nbsp;&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/allfvfmdata_thally_t300_t000.csv">allfvfmdata_thally_t300_t000.csv&nbsp;</a>. Extracted parameters are in files&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/CvaluesThally02.csv">CvaluesThally02.csv</a>,&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/psiPSI_slope_intercept.csv">psiPSI_slope_intercept.csv</a>, and&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/rP_extracted_values.csv">rP_extracted_values.csv&nbsp;</a>&nbsp;and can be analysed using the R script&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/extracted%20parameter%20plots.R">extracted parameter plots.R .</a>&nbsp;R script&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/FRRF%20visualisation%20only%20.R">FRRF visualisation only .R&nbsp;</a>&nbsp;is for visualisation only, as the title suggests.&nbsp;</p> <p>For comparing plasticity/growth , the data are in&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/20170327_giantbigtable.csv">20170327_giantbigtable.csv&nbsp;</a>, and can be visualised/analysed in&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/plast%20vs%20growth.R">plast vs growth.R&nbsp;</a></p> <p>In order to recreate the AMOVAS based on SNVs, use&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/all_variants_fixed-only_using_5x_depth_threshold.csv?versionId=01cbf6bf-fe16-4e7d-aae8-58c527596ebb">all_variants_fixed-only_using_5x_depth_threshold.cvs&nbsp;</a>&nbsp;with&nbsp;<a href="https://zenodo.org/api/files/cc14086e-84ed-4a13-b2d4-cf3cda0d669f/amova%20thally02.R?versionId=e9c21378-ee37-4418-9ce1-76db821e645a">amova thally02.R&nbsp;</a></p> <p>For additional information, please contact elisa.schaum@uni-hamburg.de&nbsp;</p>