Fig. 4 in Bat coronaviruses related to SARS-CoV-2 and infectious for human cells

Fig. 4 | BANAL-236 entryandpropagationinhumancells. a, Resultsof spike-pseudotyped BANAL-236 (squares) and SARS-CoV-2 (strain BetaCoV/ France/IDF0372/2020, GISAIDaccessionnumber EPI_ISL_406596,diamonds) pseudovirusentryassayin HEK-293T cellsexpressing (purple lines) ornot (grey lines) thehACE2 receptor,showninrelativeluminescenceunits (RLUs) producedbythefireflyluciferasepresentinthelentiviralbackbone andthe Bright-Gloluciferasesubstrate.Asingleexperimentperformedintriplicate representativeof twoexperiments isshown.Centrevaluesrepresentthe averageof thethreereplicatesanderrorbarsindicates.d. b, Resultsof spike-pseudotyped BANAL-236 (black) and SARS-CoV-2 (strain BetaCoV/ France/IDF0372/2020, grey) neutralizationassayexpressedas apercentageof neutralizationofluciferaseactivityintheabsenceof serum.Seraneutralizing SARS-CoV-2 werefrompatientswithconfirmedinfectionswhereas non-neutralizingserasampleswerecollectedbeforethespreadof SARS-CoV-2 Laos.Thedashedlinemarkstheneutralizationthreshold.Asingleexperiment representativeof threeindependentexperimentsisshown. c, Humancelllines expressingendogenous ACE2,Calu-3 (blue lines) and Caco-2 (green lines),were infectedatan MOIof 0.01 withthe BANAL-236 virus (squares) andthevirusfirst detectedin Wuhan (diamonds).VeroE6 cellswereinfectedatan MOI of 0.0001 with BANAL-236 virus (squares) and SARS-CoV-2 (strain BetaCoV/France/ IDF0372/2020, diamonds) pre-incubatedwith (grey lines) orwithout (pink lines) solublehACE2 (sACE2) at 25 Μgml−1 for 30 min. Genomecopynumberwas determinedbyquantitative RT-PCRinthesupernatantsrecovered 3 and 4 days post-infection. Asingleexperimentperformedintriplicateisshown.Centre valuesrepresenttheaverageof thethreereplicatesanderrorbarsindicates.d.