FIGURE 2 in Three interesting fungal species associated with the Asian House Gecko in Kunming, China

FIGURE 2. Phylogram generated from maximum likelihood analysis based on a combined LSU, ITS, rpb2 and mtSSU sequence datasets. Related sequences were taken from Gryganskyi et al. (2013), Nie et al. (2020) and Al-Hatmi et al. (2021). The 14 strains are included in the combined gene analyses; 3258 total characters including gaps (LSU: 1–1021 bp, ITS: 1022–1745 bp, rpb2: 1746–2590 bp, mtSSU: 2591–3258 bp). Tree topology of the ML analysis was similar to the BI. The matrix had distinct alignment patterns, with the final ML optimization likelihood value of -10673.579273 (ln). All free model parameters were estimated using the RAxML model, with 586 distinct alignment patterns and 21.73% undetermined characters or gaps. Estimated base frequencies were as follows: A = 0.280858, C = 0.195362, G = 0.257773, T = 0.266007, with substitution rates AC = 1.709550, AG = 4.063199, AT = 2.099405, CG = 1.130856, CT = 8.827188, GT = 1.000000. The gamma distribution shape parameter alpha = 0.109274 and the Tree-Length = 4.770478. The final average standard deviation of split frequencies at the end of total MCMC generations were calculated as 0.009712 in BI analysis. The species determined in this study are indicated in red. Bootstrap values equal to or greater than 70% (ML, left) and Bayesian posterior probabilities (BI, right) equal to or greater than 0.90 are given at the nodes. Hyphens (-) represent support values less than 70% in ML/0.90 in BI.