Processed Seurat Object of scRNAseq data from wildtype and CaMKK2 KO immune infiltrate of CT2a preclinical murine glioma

<p>This repository contains the processed Seurat objects generated from the raw data deposited at the Gene Expression Omnibus (GEO) under&nbsp;GSE197879.</p> <p>Details about the experiment and sequencing are available under GSE197879.</p> <p>Information on how the Seurat objects were created can be found in this GitHub repository&nbsp;https://github.com/wht10/CT2A_scRNAseq_CaMKK2KOvWT .</p> <p>Notable metadata within each Seurat object:</p> <p>1.&nbsp;Processed_CD45_Live_Fig2b.rds</p> <ul> <li>Genotype - whether the cell is from a WT or CaMKK2 KO mouse</li> <li>HTO_maxID - The biological replicate that the cell came from (4 biological replicates per genotype)</li> <li>MouseID - A concatenation between the genotype and HTO_maxID, providing a unique identifier for each biological replicate</li> <li>Cell.Type - The cell type annotations for each cell. Can be assigned to &quot;Idents()&quot; to change the name of the cell identities.</li> <li>Geno.Ident - A concatenation between Genotype and Cell.Type. By re-assigning this to &quot;Idents()&quot;&nbsp; &quot;FindMarkers()&quot; can be used to investigate differentially expressed genes within a cell-type between genotypes.&nbsp;</li> </ul> <p>2.&nbsp;Reclustered_TILs_Fig3a.rds</p> <ul> <li>Genotype - whether the cell is from a WT or CaMKK2 KO mouse</li> <li>HTO_maxID - The biological replicate that the cell came from (4 biological replicates per genotype)</li> <li>MouseID - A concatenation between the genotype and HTO_maxID, providing a unique identifier for each biological replicate</li> <li>Celltype - The cell type annotations for each cell. Can be assigned to &quot;Idents()&quot; to change the name of the cell identities.</li> <li>Geno_Ident - A concatenation between Genotype and cell-type. By re-assigning this to &quot;Idents()&quot;&nbsp; &quot;FindMarkers()&quot; can be used to investigate differentially expressed genes within a cell-type between genotypes.&nbsp;</li> </ul>