Cortical slice labelled with anti GFP and VAMP2 antibodies - sample image for software testing of "Contacting synapse" protocol

<p><strong>Image 1.tif is a Brain slice</strong>. This 16 bits confocal stack of pictures ((801x711 pixels x33 z slices - pixel size 78.17 nm) of a brain slice has been taken at 93x (LeicaHC PL APO CS2 93x/1.30 GLYC) in sequential mode with two channels : one dedicated to the GFP detection, and the other one to synpatic boutons labelled with VAMP2 protein. VAMP2 protein are expressed at glutamatergic presynaptic sites and is usually found apposed to Post Synaptic Density. This is a good sample to test &quot;contacting synapse&quot; software. Here GFP cells were electroporated with various plasmid. The aim of the software is to identify if expression of those plasmid within the GFP labelled cell, influence the density of synapse contacting this GFP cells. Here presynaptic contact are identified through the use of antibodies to VAMP2 proteins.</p>