Rhodopsin-bestrophin fusion proteins from unicellular algae form gigantic pentameric ion channels - additional data

<p>This repository stores additional data files for the article Rozenberg, Kaczmarczyk, Matzov, Vierock et al (2022) &quot;<a href="https://doi.org/10.1038/s41594-022-00783-x">Rhodopsin-bestrophin fusion proteins from unicellular algae form gigantic pentameric ion channels</a>&quot;.</p> <p>The files included are as follows:</p> <ul> <li>Inputs.zip - all input files to <a href="https://github.com/BejaLab/RRB">the workflow</a> (also available there)</li> <li>Species phylogenies: <ul> <li>Chlorophyte_orthogroups.zip, Haptophyte_orthogroups.zip, Dinoflagellate_orthogroups.zip - zip files with the orthogroups used in species phylogeny. Each folder corresponds to an orthogroup (busco orthogroups for chlorophytes and dinoflagellate, proteinortho orthogroups for haptophytes): <ul> <li>mafft.faa - mafft alignment</li> <li>trimal.faa - trimal trimmed alignment</li> <li>iqtree.treefile and iqtree.log - iqtree tree and log file</li> <li>treeshrink.treefile - treeshrink pruned tree</li> </ul> </li> </ul> </li> <li>Structural_alignment.zip includes structural alignments of the bestrhodopsin&#39;s rhodopsin and bestrophin domains with reference sequences: <ul> <li>rhodopsins.aln and bestrophins.aln- raw alignments from t_coffee</li> <li>rhodopsins_modified.fasta and bestrophins_modified.fasta - curated alignments</li> <li>rhodopsins.gff and bestrophins.gff - secondary structure features for the sequences</li> </ul> </li> <li>Global phylogeny of bestrophins and rhodopsins: <ul> <li>Bestrophins_global_sequences.zip - sequence data for the bestrophin global phylogeny: <ul> <li>uniref50.txt - uniref50 tabular data matching bestrophins (Pfam PF01062)</li> <li>ur50_long.cdhit, ur50_long.cdhit.clstr - cdhit clustering (50% identity)</li> <li>ur50_trim.faa - filtered and trimmed alignment used as input to iqtree</li> </ul> </li> <li>Bestrophins_global_phylogeny.zip - global bestrophin phylogeny. Subfolders corresponding to different runs with names corresponding to the seed values, each containing iqtree output files, in particular the newick ur50.treefile files.</li> <li>Rhodopsins_global_phylogeny.fasta, Rhodopsins_global_phylogeny.fasta.trimmed - alignment of rhodopsin sequences and its trimmed version as used for rhodopsin bestrophin phylogeny</li> <li>Rhodopsins_global_phylogeny.zip - global rhodopsin phylogeny. Subfolders corresponding to different runs with names corresponding to the seed values, each containing iqtree output files, in particular the newick rhodopsins.treefile files</li> <li>Rhodopsins_global_phylogeny_interproscan.zip - results of interproscan analysis of the rhodopsin sequences used for global phylogeny</li> </ul> </li> </ul>